10×单细胞全长转录组测序技术

快速有效的获取高质量单个RNA分子全部序列

产品介绍

皇朝国际10×DANXIBAOQUANZHANGZHUANLUZUCEXULIYONGONTZHANGDUZHANGSHISHICEXUJISHUJIEHE10XJISHUKEYIZHIJIEDUQUFANZHUANLUDEQUANZHANGcDNA,BINGJINXINGKUAISUFENXI。ZHEIYANGNENGGOUYOUXIAODEHUOQUGAOZHILIANGDEDANGERNAFENZIDEQUANBUXULIE,ZHUNQUEBIANBIEERDAICEXUWUFASHIBIEDETONGYUANYIGOUTI (isoform)、TONGYUANJIYIN、CHAOJIAZUJIYINHUODENGWEIJIYINBIAODADEZHUANLUBEN。

技术优势

细胞获取通量高

读长长

获取reads数多

单张芯片可混上万个cell

实验原理

 

皇朝国际NanoporeCEXUJIEZHUFENZITONGGUONAMIKONGSHIYINQIKONGLIANGCEDIANWEICHALAISHIXIANXINHAOJIANCE,ERATCGSIZHONGJIANJIDEDAIDIANXINGZHIBUTONG,YINCITONGGUODIANXINHAOCHAYITEZHENGJIKEJIANCECHUTONGGUONAMIKONGDEJIANJILEIXING,CONGERSHIXIANCEXU。

结果展示

1141个细胞的Illumina基因表达(h)和纳米孔转录本表达(i)的t-SNE图[2]。
差异基因(a)和差异转录本(b)的表达情况[2]

相关问题

10×单细胞全长转录组测序技术的应用方向有哪些?

XIBAOTUPUHUIZHI、SHENGWUBIAOZHIWUFENXI、ZHONGLIUYIZHIXINGYUNAIYAOFENXI、GANXIBAOFENHUAYUQIANNENGYANJIU

怎样从全血中分离 PBMC?

皇朝国际(1). JIANGQUANXUEYONG PBS 1:1 ZAIZHUIXINGGUANZHONGXISHI;

(2). ZAIXISHIDEYANGBENSHANGMIANJIARUDENGTIJIDE Ficoll;

(3). 1000g LIXIN 20min;

(4). JIANGWEIYU PBS HE Ficoll CENGJIANDE PBMC ZHUANYIDAOYIGEXINDEGUANZHONG;

(5). JIARU PBS QINGXIXIBAO;

(6). 4℃,300-400g LIXINXIBAOXUANYE 4-5min,QUCHUSHANGQINGYE;

(7). YONGBUHANGAIMEIDE 1X PBS + 0.04%BSA HUANCHONGYEZHONGXUANCHENDIANXIBAOBINGZUOJISHUHEHUOLIFENXI;

(8). ZHONGFUBUZHOU 6 HOU,JIANGXIBAOYONGBUHANGAIMEIDE 1X PBS + 0.04%BSA HUANCHONGYEZHONGXUANZHIXIBAONONGDUWEI 2×10^7/ml。

相关文献

[1]

皇朝国际Singh M, Al-Eryani G, Carswell S, et al. High-throughput targeted long-read single cell sequencing reveals the clonal and transcriptional landscape of lymphocytes. Nat Commun. 2019;10(1):3120. Published 2019 Jul 16.

[2]

皇朝国际Kevin Lebrigand, Virginie Magnone,Pascal Barbry,Rainer Waldmann.High throughput, error corrected Nanopore single cell transcriptome sequencing.November 05, 2019

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