CircRNA测序

产品介绍

HUANZHUANGRNA(circRNA)SHIYILEITESHUDEFEIBIANMARNAFENZI,YESHIRNALINGYUZUIXINDEYANJIUREDIAN。YUCHUANTONGDEXIANXINGRNA(linear RNA,HAN5’HE3’MODUAN)BUTONG,circRNAFENZICHENGFENGBIHUANZHUANGJIEGOU,BUSHOURNAWAIQIEMEIYINGXIANG,BIAODAGENGWENDING,BUYIJIANGJIE。JINJINIANDEYANJIUBIAOMING,circRNAZAISHENGWUDESHENGZHANGFAYU、DUIWAIJIEHUANJINGDEDIYUDENGFANGMIANJUYOUZHONGYAODEDIAOKONGZUOYONG。CircRNAGONGNENGZHONGDUO,KEZUOWEImiRNADEHAIMIAN,JINGZHENGXINGJIEHEmiRNA,FAHUIceRNADEDIAOKONGGONGNENG,DIAOKONGJIYINBIAODA。JINNIANLAIHAIFAXIANYUJIBINGDEFASHENGHEFAZHANMIQIEXIANGGUAN,ZAIJIBINGZHENDUANBIAOJIWUDENGFANGXIANGJUYOUHENDADEYINGYONGQIANJING。

分析内容

circrna-1

多种标准化和个性化分析

BIAOZHUNSHUJUFENXI:YUCANKAOJIYINZUDUIBI、circRNALAIYUANJIYINFENXI、YUCEcircRNA、circRNABIAODASHUIPINGFENXI

GAOJISHUJUFENXI:circRNA-miRNAHUZUOFENXI、HUZUOWANGLUOTUHUIZHI、ceRNAFENXI、WGCNAFENXI

文库类型多样化,环状/Lnc任您挑

HUANZHUANGRNAWENKUPAICHUXIANXINGRNA GANRAO,TIGAOSHUJUKEKAOXINGJILIYONGLV。lncRNAWENKUYIJUSANDE,CircRNA、mRNA、lncRNAYIWANGDAJIN,GONGBIAODAFENXISHENHUAJILIYANJIU

circrna-2

结果展示

circrna-3

CircRNA预测

circBaseSHUJUKUZHONGSHOULULEREN、XIAOSHU、XIANCHONG、MAOWEIYUHEQIANGJIYUWUGEWUZHONGDECircRNAXULIE。BAIMAIKEZONGHEMUQIANYINGYONGZUIGUANGFANDECircRNAYUCERUANJIANDUIHUANZHUANGRNACHENGHUANDEJIANJIEWEIDIANJINXINGYUCE,HOUXUANCircRNADEJIANJIEWEIDIANLIANGCECUNZAIGU/AG。XIANGTONGRANSETISHANGLAIYUANJIYINQIZHIWEIDIANXIANGTONGJIWEITONGYICircRNA。DUOGEYANGPINQUBINGJIDEDAODESUOYOUCircRNAYONGYUHOUXUFENXI。

circos分析

WOMENHUIDUICircRNAZAIJIYINZUSHANGDEFENBUZUOTONGJIFENXI,BINGYONGcircosTUCHENGXIANCHUGEGEYANGPINCircRNABIAODALIANG。circosTUQINGXIZHANSHICHUBUTONGYANGPINMOUWEIDIANHUANZHUANGRNAJIANJIEWENDIANDESHULIANGJIjunction readsFUGAISHENDU。

circrna-4
circrna-5-400400

差异CircRNA聚类分析

皇朝国际JULEIFENXIYONGYUPANDUANCHAYIJIYINZAIBUTONGSHIYANTIAOJIANXIADEBIAODAMOSHI,KETONGGUOJIANGBIAODAMOSHIXIANGTONGHUOXIANGJINDEJIYINJUJICHENGLEI,CONGERSHIBIEWEIZHIJIYINDEGONGNENGHUOYIZHIJIYINDEWEIZHIGONGNENG,TONGLEIJIYINKENENGJUYOUXIANGSIDEGONGNENGHUOGONGTONGCANYUTONGYIDAIXIEGUOCHENG。DUISHAIXUANCHUDECHAYIBIAODACircRNAZUOCENGCIJULEIFENXI,JIANGJUYOUXIANGTONGHUOXIANGSIBIAODAXINGWEIDECircRNAJINXINGJULEI。

差异CircRNA来源基因注释

SHENGWUTINEI,ZAITEDINGTIAOJIANXIAMOUXIEJIYINDUIYINGDEZHUANLUBENHUIXINGCHENGHUANZHUANGRNA,SHIQUBIANMAGONGNENG,CONGERYINGXIANGSHENGWUXUEBIAOXING。YINCI,ZHENDUIcircRNADELAIYUANJIYINFENBIEJINXINGGOHEKEGGZHUSHIJIFUJIFENXI,YOUZHUYUSHENRUWAJUEcircRNAGONGNENG。

circrna-6
没有参考基因组是否能做CircRNA呢?

皇朝国际DA:ZAICircRNAYUCESHI,CEXUclean readsXUYAOYUCANKAOJIYINZUBIDUI,DEDAOZHUNQUEDECHENGHUANJIANJIEWEIDIAN(breakpoint);RUGUOMEIYOUCANKAOJIYINZUHUOZHEZHIYOUWUCANZHUANLUZUDEDAODEUnigene,KENENGHUIYOUYUPINJIECUOWUDENGZAOCHENGCircRNAYUCEBUZHUNQUE,SUOYIWEILEDEDAOZHUNQUEDEFENXIJIEGUO,XUYAOXUANZEYOUCANKAOJIYINZUWUZHONGJINXINGFENXI。

CircRNA的成环机制都有哪些?

DA:CircRNADECHENGHUANJIZHIYOU:TAOSUOQUDONGDEHUANHUA;NEIHANZIPEIDUIQUDONGDEHUANHUA;NEIHANZIHUANHUA;RBPHUOYILEIFANSHIYINZIQUDONGDEHUANHUA;KEBIANJIANJIEYINQIDEHUANHUA。

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