真核无参转录组

产品介绍

ZHUANLUZUCEXUDEYANJIUDUIXIANGWEITEDINGXIBAOZAIMOUYIGONGNENGZHUANGTAIXIASUONENGZHUANLUCHULAIDESUOYOUmRNA。ZHENDUIWUCANKAOJIYINZUDEWUZHONG,JIANGXIAOPIANDUANPINJIECHUunigene,GOUJIANCANKAOXULIE,YIBIANHOUXUFENXI,SHIYANJIUWUCANWUZHONGFENZIJIZHIYUDIAOKONGWANGLUODEYOUXIAOSHOUDUAN。MUQIANYIGUANGFANYINGYONGYUJICHUYANJIU、LINCHUANGZHENDUAN、YAOWUYANFAHEFENZIYUZHONGDENGLINGYU。

结果展示

数据质控

皇朝国际WEIQUEBAOReadsYOUZUGOUGAODEZHILIANG,JIANGXIAJIYUANSHICEXUSHUJU(raw reads)QUDIAOHANYOUDAIJIETOUDE、DIZHILIANGDEreads,DEDAOclean reads,BAOZHENGHOUXUFENXIDEZHUNQUEXING。CEXUYINSHOUCEXUYIBENSHEN、CEXUSHIJI、YANGPINDENGYINSUYINGXIANG,CUNZAIYIDINGDECUOWULV。JIANJICEXUCUOWULVFENBUTUKEYIFANYINGCEXUSHUJUDEZHILIANG。

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测序数据组装

皇朝国际GUOLVDEDAODEGAOZHILIANGclean readsXUTONGGUOTrinityRUANJIANJINXINGZUZHUANGDEDAOZHUANLUBENXULIE。ZHUANLUBENCEXUSHENDUCHULESHOUCEXUSHUJULIANGDENGYINGXIANG,HAIYUGAIZHUANLUBENDEBIAODAFENGDUYOUGUAN。WEILESHIGEYANGPINZHONGBIAODAFENGDUJIAODIDEZHUANLUBENZUZHUANGDEGENGWANZHENG,DUIYUTONGWUZHONGDECEXUYANGPINTUIJIANHEBINGZUZHUANGKEYIJIANJIEZENGJIACEXUSHENDU,CONGERSHIZHUANLUJIEGUOGENGWANZHENG,TONGSHIYEYOULIYUHOUXUDESHUJUFENXI;ERDUIYUBUTONGWUZHONGDEYANGPIN,YOUYUJIYINZUJIANCUNZAICHAYI,TUIJIANCAIYONGFENBIEZUZHUANGHUOFENKAIFENXI。

基因表达水平分析

皇朝国际LIYONGZHUANLUZUSHUJUJIANCEJIYINBIAODAJUYOUJIAOGAODELINGMINDU。TONGGUOFPKMMIDUTUHEXIANGXIANTUBUJINKEYIFANYINGDANGEYANGPINJIYINBIAODASHUIPINGFENBUHELISANCHENGDU,HAIKEYIZHIGUANDEBIJIAOBUTONGYANGPINDEZHENGTIJIYINBIAODASHUIPINGCHAYI。

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重复相关性评估

SHENGWUXUEZHONGFUDEXIANGGUANXINGBUJINKEYIJIANYANSHENGWUXUESHIYANCAOZUODEKEZHONGFUXING,HAIKEYIPINGGUCHAYIBIAODAJIYINDEKEKAOXINGHEFUZHUYICHANGYANGPINDESHAICHA。

差异表达基因分析

CHAYIBIAODAJIYINYIHUOSHANTU、MATU、WEIENTU、JULEIRETU、DANBAIHUZUOTUDENGXINGSHICHENGXIAN,TONGGUOHUOSHANTU(Volcano Plot)KEYIKUAISUDICHAKANJIYINZAILIANGGE(ZU)YANGPINZHONGBIAODASHUIPINGDECHAYI,YIJICHAYIDETONGJIXUEXIANZHUXING。DUIYUYOUSHENGWUXUEZHONGFUDEYANGBEN,WOMENCAIYONGDEseqJINXINGYANGPINZUJIANDECHAYIBIAODAFENXI,HUODELIANGGESHENGWUXUETIAOJIANZHIJIANDECHAYIBIAODAJIYINJI;DUIYUMEIYOUSHENGWUXUEZHONGFUDEYANGBEN,SHIYONGEBseqJINXINGCHAYIFENXI。SHAIXUANCHAYIJIYINBIAOZHUNYIBANWEI:Fold Change≥2,FDR<0.01。

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差异表达基因注释及富集分析

差异表达基因GO注释分类统计图,直观的反映出在生物过程(biological process)、细胞组分(cellular component)
皇朝国际 和分子功能(molecular function),所有基因和差异基因注释GO term的个数分布。可深入挖掘差异基因的功能及所在的信号通路,筛选关注差异基因注释情况。

差异表达基因蛋白互作网络

STRINGSHOULUDUOGEWUZHONGYUCEDEHESHIYANYANZHENGDEDANBAIZHI-DANBAIZHIHUZUODESHUJUKU,BAOKUOZHIJIEDEWULIHUZUOHEJIANJIEDEGONGNENGXIANGGUAN。JIEHECHAYIBIAODAFENXIJIEGUOHESHUJUKUSHOULUDEHUZUOGUANXIDUI,GOUJIANCHAYIBIAODAJIYINHUZUOWANGLUO。

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深度数据挖掘

50+项目分析人员

丰富的项目分析案例

优化的交互式报告

8年+项目分析经验

专业而热情的售后服务

三个分布式集群服务器

闪电般的分析速度

Q1. 如何进行数据挖掘?

皇朝国际DA:KECONGSUOYOUJIYIN,CHAYIJIYINJISNPSANGEFANGMIANJINXINGSHUJUWAJUE。SUOYOUJIYINKETONGGUOGONGNENGZHUSHIXINXI,JIYINID,JIYINMINGCHENG,XULIEXINXIJIGEFANGMIANJINXINGWAJUE,TONGSHIHAIKEYIZUOBIAODAJIYINJIWEIENTU,WGCNADENGFENXI。CHAYIJIYINZEKETONGGUOWEIENTUFENXIBUTONGCHULIPICIJIGECHAYIZUHEGONGTONGDECHAYIJIYIN;TONGCHANGBIAODALIANGBIANHUAQUSHIYIZHIDEJIYIN,KENENGHUIYOUXIANGSIDEGONGNENG,GUKETONGGUOJIYINGONGBIAODAQUSHIFENXILAIJINXINGCHAYIJIYINDESHENRUWAJUE。SNPZEKETONGGUOPCAFENXI,XITONGJINHUASHU,YANGPINJIANCHAYISNPSHAIXUANJIMUBIAOQUYUSNPCHAXUNDENGJINXINGWAJUE。YISHANGZHEIXIEFENXIJUNKEZAIWOGONGSIYUNPINGTAIMIANFEIWANCHENG。

Q2. KEGG通路注释文件中,K Number Count 指的是什么?

DA: K number CountZHIXIANGGUANDEMEIDESHUMU,BIRU8(6)DAIBIAO8GEJIYINZHUSHIDAOZHEIGETONGLU,SHEJIDAOZHEIGETONGLUDE6GEMEI,MOULIANGGEJIYIN(HUODUOGE)SHEJIDAOTONGYIGEMEI。

Q3. 测序结果中GO和KEGG富集分析所用的指标分别是KS和Q-value,一般文献用的都是p value<0.05,我想问一下KS和Q-value要分别设置为多少?有没有其他测序的文章用这两个指标。

DA:GoFUJIWOMENSHIYONGDESHIBlast2GO RBAO;KEGGSHIWOMENGENJUfisherJIANYANSUANFAZIJIBIANXIEDECHENGXU。

KS<0.05,ZHEIGEZHIHEp-valueDEYIYIXIANGTONG,SHITopGORUANJIANBAOZHONGDEYIGEJIANYANFANGFA。

皇朝国际Q-value<0.01,ZHEIGEZHISHIDUIp-valueZHIDEYIGEXIAOZHENG,HEFDRGAINIANXIANGSI,SHIfisherJIANYANZHONGDEYIGEJIANYANFANGFA。

CEXUWENZHANGYIBANBUYONGZHEILIANGGEZHIBIAO,SHEJIDAOSUANFADEWENXIANZHONGCAIYOU。

Q4.假设有一个基因A,测序结果中有一部分reads可以比对到3‘和5’端,中间的部分没有序列比对上,那么有什么办法可以延伸序列鉴定到中间部分的序列。

答:(1)用实验的方法: 针对5’端和3’端的序列来设计引物,通过PCR实验进行延长和扩增.
皇朝国际 (2)生信办法: 将该基因与它的近源物种做同源,如果能找到同源基因,则将该区域的所有read比对到同源基因上,进而来确定中间部分的序列。

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