真核有参转录组

产品介绍

ZHUANLUZUCEXUDEYANJIUDUIXIANGWEITEDINGXIBAOZAIMOUYIGONGNENGZHUANGTAIXIASUONENGZHUANLUCHULAIDESUOYOUmRNA。ZHUANLUZUCEXUNENGGOUCONGZHENGTISHUIPINGYANJIUJIYINBIAODALIANGYIJIJIYINJIEGOU,JIESHITEDINGSHENGWUXUEGUOCHENGZHONGDEFENZIJILI;MUQIANYIGUANGFANYINGYONGYUJICHUYANJIU、LINCHUANGZHENDUAN、YAOWUYANFAHEFENZIYUZHONGDENGLINGYU。

结果展示

数据质控

WEIQUEBAOReadsYOUZUGOUGAODEZHILIANG,JIANGXIAJIYUANSHICEXUSHUJU(raw reads)QUDIAOHANYOUDAIJIETOUDE、DIZHILIANGDEreads,DEDAOclean reads,BAOZHENGHOUXUFENXIDEZHUNQUEXING。CEXUYINSHOUCEXUYIBENSHEN、CEXUSHIJI、YANGPINDENGYINSUYINGXIANG,CUNZAIYIDINGDECUOWULV。JIANJICEXUCUOWULVFENBUTUKEYIFANYINGCEXUSHUJUDEZHILIANG。

294
500

参考序列比对

将Clean Reads与参考基因组进行序列比对,获取在参考基因组或基因上的位置信息,定位区域分为Exon(外显子)、Intron(内含子)
和Intergenic(基因间区)。比对到参考基因组上的Reads称为Mapped Reads,Mapped Reads占Clean Reads的百分比,可以评估所选参考基因组组装是否能满足信息分析的需求。

基因表达水平分析

SHENGWUXUEZHONGFUDEXIANGGUANXINGBUJINKEYIJIANYANSHENGWUXUESHIYANCAOZUODEKEZHONGFUXING,HAIKEYIPINGGUCHAYIBIAODAJIYINDEKEKAOXINGHEFUZHUYICHANGYANGPINDESHAICHA。

609
683

重复相关性评估

皇朝国际SHENGWUXUEZHONGFUDEXIANGGUANXINGBUJINKEYIJIANYANSHENGWUXUESHIYANCAOZUODEKEZHONGFUXING,HAIKEYIPINGGUCHAYIBIAODAJIYINDEKEKAOXINGHEFUZHUYICHANGYANGPINDESHAICHA。

差异表达基因分析

CHAYIBIAODAJIYINYIHUOSHANTU、MATU、WEIENTU、JULEIRETU、DANBAIHUZUOTUDENGXINGSHICHENGXIAN,TONGGUOHUOSHANTU(Volcano Plot)KEYIKUAISUDICHAKANJIYINZAILIANGGE(ZU)YANGPINZHONGBIAODASHUIPINGDECHAYI,YIJICHAYIDETONGJIXUEXIANZHUXING。DUIYUYOUSHENGWUXUEZHONGFUDEYANGBEN,WOMENCAIYONGDEseqJINXINGYANGPINZUJIANDECHAYIBIAODAFENXI,HUODELIANGGESHENGWUXUETIAOJIANZHIJIANDECHAYIBIAODAJIYINJI;DUIYUMEIYOUSHENGWUXUEZHONGFUDEYANGBEN,SHIYONGEBseqJINXINGCHAYIFENXI。SHAIXUANCHAYIJIYINBIAOZHUNYIBANWEI:Fold Change≥2,FDR<0.01。

921
1038

差异表达基因聚类分析

皇朝国际JULEIFENXIYONGYUPANDUANCHAYIJIYINZAIBUTONGSHIYANTIAOJIANXIADEBIAODAMOSHI,KETONGGUOJIANGBIAODAMOSHIXIANGTONGHUOXIANGJINDEJIYINJUJICHENGLEI,CONGERSHIBIEWEIZHIJIYINDEGONGNENGHUOYIZHIJIYINDEWEIZHIGONGNENG,TONGLEIJIYINKENENGJUYOUXIANGSIDEGONGNENGHUOGONGTONGCANYUTONGYIDAIXIEGUOCHENG。

差异表达基因GO分类

差异表达基因GO注释分类统计图,直观的反映出在生物过程(biological process)、细胞组分(cellular component)
和分子功能(molecular function),所有基因和差异基因注释GO term的个数分布。可深入挖掘差异基因的功能及所在的信号通路,筛选关注差异基因注释情况。

1219
1333

差异表达基因蛋白互作网络

STRINGSHOULUDUOGEWUZHONGYUCEDEHESHIYANYANZHENGDEDANBAIZHI-DANBAIZHIHUZUODESHUJUKU,BAOKUOZHIJIEDEWULIHUZUOHEJIANJIEDEGONGNENGXIANGGUAN。JIEHECHAYIBIAODAFENXIJIEGUOHESHUJUKUSHOULUDEHUZUOGUANXIDUI,GOUJIANCHAYIBIAODAJIYINHUZUOWANGLUO。

常见问题

Q1. 在转录组的三个重复测序中,不是所有基因的的可变剪接都会出现3次,有的出现1次或2次,这种情况如何判定该基因是否存在相应的剪接方式?

皇朝国际DA:ZHENDUIMEIGEYANGPIN,TONGYIGEJIYINDEBUTONGZHUANLUBENHUICUNZAIKEBIANJIANJIE,WOMENZHISHIGENJUCEXUDESHIJISHUJUDUIKEBIANJIANJIEJINXINGYUCE,ERBUSHIJINXINGYANZHENG;RUGUOYAOPANDUANSHIFOUCUNZAIXIANGYINGDEJIANJIEFANGSHI,XUYAOSHIYANQUYANZHENG。ZHONGFUSHIYANCUNZAIYIDINGDECHAYI,HUIDAOZHIKEBIANJIANJIEDEBUTONG。

Q2. 转录组结果与基因组比对后,没有相应的注释结果,我们认为是New genes。但是,在NCBI注释后,有些基因的注释结果显示是参考基因组物种,这种基因还能算是new genes吗?

DA:WOMENFENXILIUCHENGZHONGSHIJIANGCEXUDEReadsBIDUIDAOCANKAOJIYINZU,RANHOUJINXINGPINJIE,QIZHONGYIXIEreadsBIDUIDAOJIYINJIANQUBINGQIENENGPINJIECHUWANZHENGDEKAIFANGYUEDUKUANG,PINJIECHULAIDEWEIYUJIYINJIANQUDEZHEIXIEJIYINJIWEIXINJIYIN。YUCEDEDAODEXINJIYINCAIHUIJINXINGGONGNENGZHUSHI,SUOYIZHUSHIJIEGUOYUXINJIYINDEPANDUANMEIYOUGUANXI。

Q3:生物学重复样品中某个样品与其他相关性不太好该怎么处理?会不会影响文章发表?

皇朝国际DA:WEILEQUEBAOFENXIJIEGUODEZHUNQUEXING,LAOSHITONGCHANGHUISHEZHI3GESHENGWUXUEZHONGFU,ZHEIYANGJIUKENENGCHUXIANSHENGWUXUEZHONGFUZHONGMOUGEYANGPINXIANGGUANXINGBUHAODEQINGKUANG,YINGXIANGHOUXUCHAYIFENXIJIEGUODEZHUNQUEXING。TONGCHANGKEJIANGGAICHULIZUZHONGXIANGGUANXINGBUHAODEYANGPINTICHU,ZAIJINXINGCHAYIFENXI。HOUQIKETONGGUORT-qPCRDENGSHIYANSHOUDUANMIBUSHENGWUXUEZHONGFUDEBUZU,BUHUIYINGXIANGWENZHANGDEFABIAO。

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