遗传图谱

JINGDIANDEYICHUANXUEYANJIUFANGFA

YICHUANTUPUSHIYIJURANSETIJIAOHUANYUZHONGZU,YIDUOTAIXINGDEYICHUANBIAOJIWEILUBIAO,YIBIAOJIJIANDEZHONGZULVWEI“TUJU”,

QUEDINGBUTONGDUOTAIXINGBIAOJIWEIDIANZAIMEITIAOLIANSUOQUNSHANGPAILIEDESHUNXUHEYICHUANJULIDEXIANXINGLIANSUOTUPU

high-density-genetic-map

YINGYONGLINGYU

qtl__
QTL定位
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标记辅助育种
_
辅助基因组组装
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比较基因组

ZHENDUIXINGDEGEXINGHUAFANGAN

 CONGWUZHONGGETIQUYANGDAOHOUXUANJIYINWAJUE,WOMENJIANGWEININTIGONGYIZHANSHIGEXINGHUAJIEJUEFANGAN,RANGFENXIGUOCHENGKUAISU、KEXUE、QINGXI!

  • 物种取样

  • 建库测序

  • 标记分型

  • 图谱构建

FENXINEIRONG

皇朝国际CEXUSHUJUXIAJIHOU,MEIYIGEFENXIGUOCHENGWOMENDOUYANGEBAKONG,ZHENDUIBUTONGDECEXUFANGSHI,CONGSHUJUZHIKONGDAOYICHUANTUPUDEGOUJIAN,YOUZHUANMENDEDELIUCHENGJINXINGFENXI。

全基因组重测序
测序数据质控
与参考基因组比对
变异检测及注释
多态标记开发
遗传图谱构建及评估

简化基因组测序(SLAF)
测序数据质控
与参考基因组比对(有参)
标签聚类比对(无参)
SNP变异检测及注释
多态标记开发
遗传图谱构建及评估

SLAFJIANHUAYICHUANTUPU

SLAF-seqJISHUSHIBAIMAIKEGONGSIZIZHUYANFADEYITAOJIYUMEIQIEDEJIANHUAJIYINZUCEXUCELVE,

BUSHOUCANKAOJIYINZUXIANZHI,NENGGOUYOUXIAODIJIANGDIWUZHONGJIYINZUFUZADU,

皇朝国际SLAFBIAOQIANGOUJIANYICHUANTUPUJUYOUSHANGTUBIAOJISHULIANGDUO、TUPUZHILIANGGAO、ZHOUQIDUAN、SHUJULIYONGLVGAODENGDEYOUDIAN。

适用人群

YOUWUCANKAOJIYINZUJUNKE

测序平台

IlluminaPINGTAI PE150CEXU

项目周期

70GEGONGZUORI

ZHONGCEXUYICHUANTUPU

皇朝国际DUIYIYOUCANKAOJIYINZUXULIEDEWUZHONGJINXINGQUNTIQUANJIYINZUZHONGCEXU,LIYONGGAOXINGNENGJISUANPINGTAIHESHENGWUXINXIXUEFANGFA,

皇朝国际JIANCEDANHEGANSUANDUOTAIXINGWEIDIAN(SNP),BINGJISUANDUOTAIXINGBIAOJIJIANDEYICHUANLIANSUOJULI,

HUIZHIGAOMIDUDEYICHUANTUPU, ZAIJINXINGQTLDINGWEIZHONG,KEZHIJIEHUOQUDINGWEIQUJIANNEIDEXULIEXINXI,JIAKUAIGONGNENGJIYINDEJIANDINGHEYANZHENGDEJINDU

适用人群

YOUCANKAOJIYINZUWUZHONG

测序平台

皇朝国际IlluminaPINGTAI PE150CEXU

项目周期

90GEGONGZUORI

BAIMAIKEYOUSHI

36020170502173230245_

ZIZHUZHISHICHANQUANJIANHUAJIYINZUCEXUJISHUSLAF-seq

ZHUANMENZHENDUIGAOTONGLIANGCEXUKAIFADEHighMapZUOTURUANJIAN

皇朝国际FENGFUDEXIANGMUJINGYAN,YIWANCHENGBAIYUGEWUZHONGCHAOGUOJINQIANZHANGGAOMIDUYICHUANTUPU

JIEZHIMUQIANYIFABIAOSCIWENZHANG150+PIAN,LEIJIYINGXIANGYINZI650+

皇朝国际GONGSIYONGYOU 40 YUXIANGZHUANLIJISHUHE 150 YUXIANGRUANJIANZHUZUOQUAN,WEIFENXIJIEGUOBAOJIAHUHANG

CHENGGONGANLI

测序数据统计与评估

GAOZHILIANGDECEXUSHUJUSHIJINXINGHOUXUFENXIDEZHONGYAOJICHU,ZAIHUODECEXUDERaw dataHOU,WOMENJIANGHUIJINXINGCEXUSHUJUCHANCHUTONGJI、JIANJICEXUZHILIANGFENBU、JIANJILEIXINGFENBUDENGDUOZHONGCEXUPINGGUFANGSHI,BAOZHENGCEXUSHUJUDEZHUNQUEXING。

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#Chr Pos Ref R01 R02
Chr1 113342 A G A
Chr1 163871 A G A
Chr1 232230 A A G
Chr1 232330 C C A
Chr1 232546 T T C

群体遗传变异检测

SNP(Single Nucleotide Polymorphism)JIInDel(Insertion-Deletion)SHIJIYINZUSHANGZUIWEICHANGJIANDEYICHUANBIAOJI,JUYOUSHULIANGDUO、DUOTAIXINGFENGFUDETEDIAN,SNPHEInDelDEJIANCEZHUYAOTONGGUOGATKHEsamtoolsRUANJIANGONGJUBAOSHIXIAN。BIANYIWEIDIANZHUSHISHIYONGSnpEffRUANJIAN。

多态标记开发

皇朝国际ZAIBIANYIWEIDIANJIANCEDEJICHUSHANG,KAIFAQINBENJIANDEDUOTAIXINGBIAOJI,BINGDUIZIDAIZHONGZHEIXIEDUOTAIXINGBIAOJIJINXINGJIYINXINGFENXING。

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_

遗传图谱构建

皇朝国际WEIBAOZHENGYICHUANTUPUZHILIANG,DUIZIDAIZHONGDUOTAIXINGBIAOJIJINXINGYANGEDESHAIXUANGUOLV,BAOKUOQINBENSHENDUGUOLV、YICHANGWEIDIANGUOLV、BIAOJIWANZHENGDUGUOLVJIPIANFENLIBIAOJIGUOLVDENG,HUODEGAOZHILIANGBIAOJI。LIYONGBAIMAIKEZIZHUYANFADEHighmap-mapRUANJIANJINXINGGAOMIDUYICHUANTUPUDEHUIZHI。

遗传图谱评估

皇朝国际WEILEBAOZHENGYICHUANTUPUDEZHILIANG,BAIMAIKECAIYONGLEMUQIANZUIWEIYANGEDETUPUPINGJIATIXI,BAOKUOSHANGTUBIAOJIWANZHENGDUTONGJI、DANTILAIYUANPINGGU、LIANSUOGUANXIPINGGU、YICHUANTUPUGONGXIANXINGFENXIDENG。

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不同物种如何推荐作图群体?

皇朝国际GENJUQINBENZAHE、CHUNHEQINGKUANGXUANZEFENLIQUNTILEIXING:

(YI)QINBENZAHEDEWUZHONG,RULINMU、SHUICHAN、GUOSHUDENGYIHUASHOUFEN、CHONGMEI、FENGMEISHOUFEN、CIXIONGYITIWUZHONG,YIBANCAIYONGF1。

皇朝国际(ER)QINBENCHUNHEWUZHONG,RUSHUIDAO、DOULEIDENGZIHUASHOUFEN,FENLIQUNTIKEYONGF2、BC、RIL、DH、CHAOSHIQUNTIDENG。

重测序遗传图亲本及子代测序深度推多少合适?

LIANGGEQINBENTUI10-20×,ZIDAICEXUSHENDUKANWUZHONGJIYINZUZUZHUANGCHENGDU,RUGUOZUZHUANGJIEGUOBUHAODEHUA,DABUFENPIANDUANDOUBIJIAODUAN,KENENGZUIHOUHUIYINGXIANGYICHUANTUPUDEJIEGUO,TUIJIANCEXUSHENDU3XYISHANG,RUGUOJIYINZUZHUANGDAORANSETISHUIPINGHUOZHEN50DADAOMbJIBIECE1×JIUKEYILE。

亲本和参考基因组是一个品种,比如水稻构建群体的亲本是9311,是否需要重新对亲本进行测序?

XUYAO,9311JIYINZUSUIRANCHULAI,DANSHI9311YOUHENDUOBUTONGLEIXINGDETUBIAN,BIRUYOUDEYOUMANG,YOUDEWUMANG,JIANYILAOSHICE,DANSHICEXUSHENDUKEYISHAOWEIJIANGDI。

遗传图表型数据是否必须满足正态性才能进行QTL性状定位?

皇朝国际DABUFENQTLDINGWEIFANGFAZHISHIYAOQIUBIAOXINGSHUJUZHONGDESUIJIWUCHAXIANGFUCONGZHENGTAIFENBU,BINGMEIYOUYAOQIUBIAOXINGSHUJUMANZUZHENGTAIFENBU。SHULIANGXINGZHUANGZHIYOUZAIDUOJIYINJIASHUOXIACAIZHENZHENGFUHEZHENGTAIFENBU,BIAOXINGSHUJUDEFEIZHENGTAIXINGBINGBUYINGXIANGQTLDINGWEI。

上图标记中是否包含偏分离标记?偏分离标记原因是什么?

BAOHANPIANFENLIBUXIANZHUDEBIAOJI,XIANZHUPIANFENLIDEBIAOJIHUIBEIGUOLVDIAO。PIANFENLISHIZIRANJIEFEICHANGPUBIANCUNZAIDEXIANXIANG,BINGBEIRENWEISHISHENGWUJINHUADEDONGLIZHIYI。CHANSHENGPIANFENLIDEYUANYINZHUYAOYOULIANGGEFANGMIAN:PEIZIXUANZEHEHEZIXUANZE,QIZHONGPEIZIXUANZEZHUYAOBAOKUOHUAFENZHISI、HUAFENGUANJINGZHENGHEXUANZEXINGSHOUJING。CHULESHANGSHUYUANYINYIWAI,HUANJINGYINSU、FEITONGYUANZHONGZU、JIYINZHUANHUAN、ZHUANZUOYINZI、ZHUANJIYINCHENMO、TIWAIGUXIONGSHENGZHIGUOCHENGZHONGDEXUANZEYA、FEIZHENGBEITIHUOBUWENDINGYIWEIZAOCHENGDERANSETIBUWENDINGDENGDOUYOUKENENGSHIDAOZHIPIANFENLI。

高密度遗传图是如何辅助基因组组装的?

皇朝国际TONGGUOGONGXIANXINGFENXI,JIANGYICHUANTUPUSHANGDEBIAOJIYUCHUBUZUZHUANGDEDAODEscaffoldJINXINGBIDUI,TONGJIBUTONGscaffoldSHANGDEBIAOJISHUMU,DEDAOBUTONGLIANSUOQUNSHANGBEIMAODINGDEscaffoldXINXI,YIGEscaffoldSHANGRUGUOMAODINGLIANGGEYISHANGDEBIAOJI,GAIscaffoldDEWEIZHIHEFANGXIANGJUNKEYIBEIDINGWEIDAORANSETISHUIPING。ZHIYOU1GEBIAOJIZEZHINENGDINGWEIDAOWEIZHI,FANGXIANGBUNENGQUEDING。RUGUOMEIYOUMAODINGDAOBIAOJI,ZEGAIscaffoldRENGRANBUNENGBEIDINGWEIDAORANSETISHUIPING,SHUOMINGGAIscaffoldDUOTAIXINGKENENGJIAOCHA,XUYAOGOUJIANGENGJIAJINGXIDEYICHUANTUPU。

过滤偏分离标记0.001和0.01哪个更严格?

皇朝国际PANDUANBIAOJISHIFOUPIANFENLISHICAIYONGDEKAFANGJIANYAN,KAFANGJIANYANDEXIANZHUXINGPZHIYUEXIAO,YUEZHENGMINGGAIBIAOJIPIANFENLIYANZHONG,YINCIPZHIXIAOYU0.001DEBIAOJIYAOSHAOYUPZHIXIAOYU0.01DEBIAOJIDESHULIANG。ZAIJINXINGGUOLVDESHIHOURUGUOANZHAOPZHI<0.001GUOLV,SHENGYUDEBIAOJIHUIGENGDUO,YINCISHIBIJIAOKUANSONGDEGUOLVBIAOZHUN,FANZHI,ANZHAOPZHI<0.01GUOLV,HUIGUOLVDIAOGENGDUODEBIAOJI,SHIGENGWEIYANGEDEGUOLVBIAOZHUN。

用F2的DNA来做图谱,用F2:3来做性状调查,问一下这个怎么做?

QUF2DEYEPIAN,F2:3JIAXIDEBIAOXINGPINGJUNZHIZUOWEIF2DEBIAOXING,QUNTIYAOSUIJIQUYANG(F2:3JIAXISHIZHIF2DAIQUNTIZHONG,MEIGEF2DAIZHIZHUZIJIAOYOUCHANSHENGDEGEZIDEF3QUNTIZUCHENGDEQUNTI,ZHEIGEF3SHIYUSHANGYIDAIF2SUODUIYINGDE,SUOYIYECHENGWEIF2:3JIAXI,TAMENSHIBUNENGHUNZAIYIQIDE,HUNZAIYIQIJIUJIAOF3LE,ERBUSHIF2:3。ZHEIZHONGQUNTIZHUYAOSHIYONGYUCHUBUDINGWEIDE,DUIYUDANKONGZHIDEZHILIANGXINGZHUANGDINGWEIXIAOGUOHENHAO。)

亲本重测序,子代简化作图有何优势?

WUXUJIANLIBACKU,QINBENZHONGCEXUKEYIHUODEQTLQUYUDEJIYINXULIE,JINXINGJIYINYUCEHEHOUXUBIAOJIKAIFAZHUANJIYINYANZHENGDENGGONGZUO。

什么是eQTL,和基于DNA水平开发SNP的区别?

1)BIAODASHULIANGXINGZHUANGJIYINZUO(expression Quantitative Trait Loci,eQTL)SHIZHIDESHIRANSETISHANGYIXIENENGTEDINGDIAOKONGmRNAHEDANBAIZHIBIAODASHUIPINGDEQUYU,QImRNA/DANBAIZHIDEBIAODASHUIPINGLIANGYUCHENGBILIGUANXI,JIANGGEJIYINXINGDEBIAODASHUJUZUOWEIYIGESHULIANGXINGZHUANG,LIYONGCHUANTONGDEQTLFENXIFANGFAJINXINGFENXI。eQTLKEFENWEIeQTLHEeQTL,SHUNSHIZUOYONGeQTLJIUSHIMOUGEJIYINDEeQTLDINGWEIDAOGAIJIYINSUOZAIDEQUYU,BIAOMINGKENENGSHIGAIJIYINBENSHENDECHABIEYINQIDEmRNASHUIPINGBIANHUA;FANSHIZUOYONGeQTLSHIZHIMOUGEJIYINDEeQTLDINGWEIDAOQITAJIYINZUQUYU,BIAOMINGQITAJIYINDECHABIEKONGZHIGAIJIYINmRNASHUIPINGDECHAYI。

2)JIYUZHUANLUZUCEXUDEGUANLIANFENXISHICONGSNPGUANLIANFENXIYUGEMGUANLIANFENXILIANGGESHUIPINGSHIXIANDE,KAIFADESNPWEIYUJIYINBIANMAQUYU,SNPYUJIYIN(HUOUnigene)SUOSHUGUANXISHIYIZHI,JIASHANGJIYINBIAODASHUIPINGDEGUANLIANFENXIJIUNENGGOUSHIXIANGONGNENGJIYINDEJINGXIDINGWEI。

公司常用的作图软件有哪些?

ZHUYAOYOUSIKUAN:R-qtl、MapQTL、WinQTLcartHEICiMapping。

为什么遗传图亲本测序量要比子代高?

ZHEISHIYOUYUBIAOJIDEZIDAIFENXINGYILAIYUQINBENDEFENXINGZAOCHENGDE。YICHUANTUZHONGMEIYIGEBIAOJIDEZIDAIFENXINGSHOUXIANYAOGENJUQINBENDEFENXINGLAIJINXINGFENXINGPANDUAN,YINCIQINBENFENXINGDEZHUNQUEXINGJIUHENZHONGYAO,SHIYIKAOGAOSHENDUDECEXULAIQUEBAOQINBENFENXINGDEZHUNQUEXING,YINCIZAICEXUDESHIHOUYAOBIZIDAIDECEDEDUO。

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