空间转录组测序

定位和区分功能基因在特定组织区域内的活跃表达

产品介绍

KONGJIANZHUANLUZUSHICONGKONGJIANCENGMIANSHANGJIEXIRNA-seqSHUJUDEJISHU,CONGERJIEXIDANGEZUZHIQIEPIANZHONGDESUOYOUmRNA,CONGERNENGGOUDINGWEIHEQUFENGONGNENGJIYINZAITEDINGZUZHIQUYUNEIDEHUOYUEBIAODA。

空间转录组技术原理

皇朝国际1、DUIXINXIANLENGDONGZUZHIJINXINGQIEPIAN,BINGJINXINGCHENGXIANG。

2、JIANGZUZHIQIEPIANFANGZHIZAIHANYOUYURNAJIEHEBUHUOTANZHENDEZAIBOPIANSHANG,BINGJINXINGGUDINGHETOUHUA(SHIXIBAOZHONGDEmRNADEDAOSHIFANG,BINGJIEHEDAOXIANGYINGDEBUHUOTANZHENSHANG,CONGERHUOQUJIYINBIAODAXINXI)。

3、YIBUHUODERNAWEIMOBANJINXINGcDNAHECHENGHECEXUWENKUZHIBEI。

4、DUIZHIBEIHAODEWENKUJINXINGCEXU。

5、SHUJUKESHIHUAFENXI(QUEDINGNAXIEJIYINDEDAOBIAODA,JIYINBIAODALIANG,YIJIZHEIXIEJIYINDEKONGJIANWEIZHIXINXI)。

10x Genomics空间转录组技术原理

 

10x GenomicsKONGJIANZHUANLUZUYONGYUWENKUGOUJIANDEMEIZHANGZAIBOPIANSHANGYOUSIGEBUHUOQUYU,MEIGEBUHUOQUYUDEDAXIAOWEI6.5×6.5mm,BAOHAN5000GEBEITIAOXINGMABIAOJIDEDIAN(barcoded spots),MEIGEDIANDEZHIJINGWEI55 μm,DIANHEDIANZHIJIANZHONGXINDEJULIWEI100 μm,BINGQIEMEIGEDIANDOUYOUYIGEWEIYIDEbarcodeXULIE。

ZUZHIQIEPIANDEXIBAOZHONGHUISHIFANGCHUmRNA,QIANYIDAOMEIGEspotDEmRNAHUIBEIBIAOJISHANGXIANGYINGDEbarcodeXULIE,RANHOUJINXINGWENKUGOUJIANBINGJINXINGCEXU。

皇朝国际ZUIHOU,GENJUSHUJUDETIAOXINGMAXINXIDUISHUJUJINXINGFENXI,YIQUEDINGNAXIESHUJULAIZINAGEWEIZHI,CONGERSHIXIANKONGJIANJIYINBIAODADEKESHIHUA。

 应用方向

  • 病理学

    TONGGUOTIANJIAJIYINBIAODAWEIDU,DECHUXINGTAIXUEJIELUN

  • 免疫学

    皇朝国际MIANYIXIBAOJINRUN、MIANYIXIBAOZHONGDEJIYINBIAODATEZHENG

  • 发育生物学

    FAXIANZUZHIBEIJINGZHONGYUXINGTAIXINGCHENGYOUGUANDEJIYIN

  • 神经科学

    HUIZHIDANAODEGEGEXIBAOCENG、QUFENZHENGCHANGYUHUANBINGDEDANAOJIEPOUTEZHENG

  • 肿瘤学

    ZHONGLIUWEIHUANJING、ZHONGLIUYIZHIXING、BINGCHENGJINZHAN、AIZHENGXINGTAI、ZHONGLIUJINRUNLINBAXIBAO

结果展示

 小鼠肾脏空间转录组的mRNA表达及聚类

皇朝国际ADAIBIAOZUZHIQIEPIANDEHERANSE。BHECFENBIEDAIBIAOUMIJISHUHEZONGJIYINJISHUYUZUZHIKONGJIANWEIZHIDETUXIANGDIEJIA。DDAIBIAOJIYUZONGTICHAYIBIAODAJIYINDEKONGJIANJULEIJIEGUO,ZUIYOUBIANXIANSHILEcluster2PAIMINGQIAN10DETEYIBIAODAJIYIN。E、F、G、HFENBIEDAIBIAOLEBUTONGJIYINDEKONGJIANmRNABIAODA。

小鼠大脑空间分辨率的基因表达

皇朝国际ADAIBIAOZUZHIQIEPIANDEHERANSE。BHECFENBIEDAIBIAOHAIMAZUZHIDEBIAOJIJIYINTmsb4xHESelenowJIYINDEKONGJIANBIAODAQINGKUANG,JIEGUOXIANSHIZHEILIANGGEJIYINZAIHAIMATIZHONGXIANZHUGAOBIAODA,FUHEYIZHIDEJIYINBIAODAMOSHI

常见问题

数据量如何确定

皇朝国际YANGBENLEIXING、Coverage、BIAODALIANGBUTONG、SHUJULIANGYEHUIBUTONG,SHUJULIANGTUIJIAN≥50k reads pairs per spot,(50% tissue coverage * 5000 total spots) * 50,000 read pairs per spot= 125 million read pairs (250M total reads)。GU,TUIJIANSHUJULIANG30G-60G/YANG

空间转录组数据如何可视化?

XIANGQINGJIAN:

样本如何制备?

SHIYANLIUCHENGWEI:XINXIANZUZHIQUYANG-YIWUWANDONGCUN-OCTBAOMAI-LENGDONGQIEPIAN-RANSE-XIANWEIJINGGUANCHA-JIANKUCEXU XUYAOKEHUZHUNBEI:XINXIANZUZHIQUYANG–YIWUWANDONGCUN(BAOCUNHUOYUNSHU)–OCTBAOMAI–BAOCUNHUOYUNSHU KEHUZAIZHIBEIYANGBENSHI,XUYAOZHUNBEI3FENYANGBEN(ZHISHAO2FEN),MEIKUAIZUZHIYANGBENBUYIGUODA(ZHANGKUANGAOYUE0.8 cm )

a.YIFENYONGYUCHANGGUIRNATIQU,YIQUEBAOYANGBENZISHENJIYANGBENZHIBEIBUCUNZAIWENTI,DANGRNA RIN>8SHI,KEJINXINGHOUXUSHIYAN

b.YIFENYONGYUZHENGCHANGDEQIEPIANSHIYAN

c.YIFENYONGZUOBEIFEN

XIANGQINGJIAN:

空间转录组的已发表文献有哪些?

标题

时间 期刊 题目 IF 类别
2020.01 Nature Biotechnology Integrating microarray-based spatial transcriptomics and single-cell RNA-seq reveals tissue architecture in pancreatic ductal adenocarcinomas 31.864​ PDAC肿瘤异质性
2020.01 Nature cell biology Combined single-cell and spatial transcriptomics reveal the molecular, cellular and spatial bone marrow niche organization 17.428 骨髓造血微环境
2019.12 Cell A Spatiotemporal Organ-Wide Gene Expression and Cell Atlas of the Developing Human Heart 36.216 人类心脏发育
2019.04 Science Spatiotemporal dynamics of molecular pathology in amyotrophic lateral sclerosis 41.037 肌萎缩性侧索硬化症
2018.11 Nat Protoc Barcodedsolid-phase RNA capture SpatialTranscriptomics profiling mammaliantissue sections 11.334 实验方法
2018.10 Cancer Research Spatially Resolved Transcriptomics Enables Dissection of Genetic Heterogeneity in Stage III Cutaneous Malignant Melanoma 8.378 黑色素瘤异质性
2018.06 Nat Commun Spatial maps of prostate cancer transcriptomes reveal an unexplored landscape of heterogeneity 11.878 前列腺癌异质性

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