ATAC-seq

产品介绍

ATAC-seq(Assay for Transposase-Accessible Chromatin with highthroughput sequencing),JIYUGAOTONGLIANGCEXUDERANSEZHIZHUANZUOMEIKEJIEJINXINGSHIYAN。ZAIHEXIAOTILIANJIEZHIMIDEDIFANG,ZHUANZUOMEIBUNENGJINRU,ERSONGSANDEQUYU,ZHUANZUOMEINENGGOUJINRUBINGQIEGEXIABAOLUDEDNAQUYU,BINGTONGSHILIANJIESHANGTEYIXINGDEJIETOU,YOUJIETOUDEDNAPIANDUANBEIFENLICHULAI,YONGYUGAOTONGLIANGCEXU。YINCI,ATAC-seqKEYIDESHIQUANJIYINDUCHIDUSHANGCHUYUKAIFANGZHUANGTAIDERANSEZHIQUYU,BINGQIETONGGUOFENXIRANSEZHIKAIFANGQUYUDEmotif,KEYIHUODEQIANZAIDEHUOYUEZHUANLUYINZIJIQIBAJIYIN。

服务流程

  • 样品寄送

  • 建库测序

  • 数据分析

  • 出具报告

  • 售后答疑

实验流程

ATAC-seqZHUYAOSHIYANLIUCHENGSHOUXIANSHIZHIBEIXIBAOXUANYE,RANHOULIEJIEYELIEJIEXIBAOHUODEXIBAOHE,JINYIBUSHIYONGTn5ZHUANZUOMEIMEIQIEBINGCHUNHUA,ZUIHOUHUISHOUDNAPIANDUAN,DUIHUISHOUPIANDUANJINXINGMODUANXIUFU、3’DUANJIAA、LIANJIECEXUJIETOU,PIANDUANDAXIAOXUANZEYIJIPCAKUOZENGDENGBUZHOUHUODEBIAOZHUNDEGAOTONGLIANGCEXUWENKU,SHIYONGIlluminaCEXUPINGTAIJINXINGCEXU。

信息流程

YUANSHICEXUReadsJINGGUOQUJIETOUHEGUOLVDIZHILIANG,HUODEClean Reads。JIANGClean ReadsYUCANKAOJIYINZUXULIEJINXINGBIDUI,TONGGUOReadsZAIJIYINZUSHANGDEBIDUIWEIZHIXINXI,JINXINGPeak calling,BINGJINXINGmotifYUCE、ZHUANLUYINZIBAJIYINWAJUE、CHAYIPeakHEMotifDENGFENXI。

2
开放染色质区域可视化图
3
转录起始位点附近的开放染色质区域
4
差异开放染色质区域聚类热图
5
开放染色质motif
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差异开放染色质区域功能基因富集分析

 常见问题

ATAC-seq需要生物学重复吗?

XUYAODE,TUIJIAN3-5GEZHONGFU,MUDESHIWEILELAOSHISHIYANJIEGUODEZHUNQUEXINGHEKEZHONGFUXING。

到底motif是什么?

JIANDANDIJIANG, Motif JIUSHIYIDUANTEDINGMOSHIDEDNAXULIE。KEYILIJIEWEIKAIFANGDERANSEZHIQUYUYOUZHEXULIEPIANHAOXING,ERPIANXIANGDEXULIEJIUSHImotif。

ATAC-seq和Chip-seq的区别?

Chip-seqSHIYANJIUTEDINGZHUANLUYINZIHUOZHEDANBAIJIEHEDEQUYU,XUYAOYONGKANGTIQUMIANYIGONGCHENDIANXIANGYINGDEQUYU。ERATAC-seqWUXUKANGTI,KEJIANGSUOYOUZHUANLUYINZIHUOZHEDANBAIKENENGJIEHEDEQUYUYONGTn5ZHUANZUOMEIQIEGEXIALAIJINXINGCEXU。

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