医学Hi-C互作测序

产品介绍

MUQIAN,DUIJIYINBIAODADIAOKONGDEYANJIUZHUYAOSHIYIJIYINJIQIDIAOKONGYUANJIANDEXIANXINGGUANXIWEIJICHU,RANER,JIYINBUJINJINYIJIANDANDEXIANXINGXINGSHICUNZAI,YUELAIYUEDUODEZHENGJUBIAOMINGRANSEZHIKONGJIANHUZUOZAIJIYINBIAODADIAOJIEFANGMIANYEQIZHONGYAOZUOYONG,JIJIYINDEBIAODADIAOKONGCUNZAISANWEIKONGJIANWANGLUO,JIYINBIAODAKEBEIYUANCHENGDIAOKONGYUANJIANSUODIAOKONG。RANSEZHIDEKONGJIANJIEGOU,YEJIRANSEZHIDESANWEIGAOJIJIEGOUYUGONGNENG,SHIBIAOGUANYICHUANXUEDEYIGEXINXINGDEZHONGYAOYANJIULINGYU。

Hi-C(High-throughput chromosome conformation capture)SHIRANSEZHIGOUXIANGBUHUOJIEHEGAOTONGLIANGCEXUYANSHENGDEYIZHONGJISHU,SHIXIANLEQUANJIYINZUFANWEINEIDERANSETIPIANDUANJIANXIANGHUZUOYONGDEBUHUO。Hi-CHUZUOZHUYAOSHIJIANGKONGJIANJIEGOULINJINDEDNAPIANDUANJINXINGJIAOLIAN,BINGFUJIJIAOLIANDEDNAPIANDUAN,RANHOUJINXINGGAOTONGLIANGCEXU,DUICEXUSHUJUJINXINGFENXIJIKEJIESHIRANSETIPIANDUANJIANDEJIAOHUZUOYONG,CHANSHURANSETISANWEIGOUXIANG,GUANGFANYINGYONGYUZHONGLIU/JIBINGFASHENGFAZHANJIZHI、FENHUAFAYUJIZHI、JIBIANJIZHIDENGYANJIU。

技术路线

A/B compartments

A compartments:常染色质,松散染色质状态、高基因密度、转录活跃区域,富集转录活性相关的表观遗传标记(例如H3K4me3)。
B compartments:异染色质,压缩染色质状态、低基因密度区域、转录抑制区域,富集无转录活性的表观遗传标记(例如H3K27me3)。

TUOPUXIANGGUANJIEGOUYU(TAD)

皇朝国际TADSHIYIDUANJUYOUZHEDIEJIEGOUDEDNAXULIE,QINEIBUDEHUZUOPINLVXIANZHUGAOYUPILINQUYUZHIJIAN。TADBIANJIEYOUMINGXIANDEJIEXIAN,NEIBUSHIYIGEDULIDEDIAOKONGDANYUAN,NEIBUDEJIYINCUNZAIXIETONGBIAODATEZHENG。

RANSEZHIHUAN(loop)

皇朝国际loopSHIZHITONGGUOCTCFDENGDANBAIZHIJIEDAOXINGCHENGDEYUANJULIHUZUODNAPIANDUANERMAODINGXINGCHENGHUANZHUANGJIEGOU,loopMAODINGWEIDIANZHONGCHANGFUHANQIDONGZI、ZENGQIANGZI、CHENMOZIDENGDIAOKONGYUANJIAN。

皇朝国际SHENGWUTIBUTONGFAYUSHIQI, YIJIBUTONGDEXIBAOLEIXINGZHONG, RANSEZHIDESANWEIKONGJIANJIEGOUSHIDONGTAIBIANHUADE,RUA/B compartmentZHUANHUAN,TADBIANJIEBIANHUA,loopXIAOSHIHUOXINXINGCHENG,ZHEIZHONGSANWEIGOUXIANGDEBIANHUAYUJIYINBIAODADIAOKONGMIQIEXIANGGUAN。

应用方向

Hi-C多组学研究

 结果展示

A/B compartment及转换分析

1
2

TAD及差异分析

tad
TAD全基因组分布图
7
差异TAD边界DI值聚类图
8
差异TAD边界关联的差异表达基因聚类图

Loop及差异分析

9
Loop可视化热图
10
全基因组loop分布图
11
Loop anchor 位点分析
12
差异Loop聚类热图
13
差异Loop相关DEG KEGG通路分析

 常见问题

Hi-C互作测序应该做多少数据量?

ZHUYAOKANSHIFOUGUANZHUloopSHUIPINGFENXI(ZENGQIANGZI-QIDONGZIHUZUODENG),GUANZHUloopSHUIPING,BAIMAIKETUIJIAN150XJI450GSHUJULIANG,DADAO10kbYIXIAFENBIANLV,TONGSHIFENXIA/B compartment、TADHEloop;RUOBUGUANZHUloopSHUIPING,ZHIGUANZHUcompartmentHETAD,ZEKEYIZUO50X(150GSHUJULIANG)。DANGRAN,RUGUOGUANZHUGENGGAOFENBIANLV,RU5kbZEXUYAO900GbSHUJULIANG(300X),FENBIANLVYUEGAO,SUOXUYAOSHUJULIANGYUEDA。

Hi-C互作测序是否需要设置生物学重复?

Hi-CHUZUOZHUYAOJIEXIDESHIRANSEZHISANWEIGOUXIANGDUIYUJIYINBIAODAJIBIAOXINGXINGZHUANGDENGDIAOKONGJIZHI,JIANYIZHISHAOSHEZHI2GESHENGWUXUEZHONGFU。DUIYUXIBAOXIYANGBEN,KEYIKAOLVMEIZU3GESHENGWUXUEZHONGFU,MEIGEZHONGFU50XJI150GbSHUJULIANG,HEBINGGEZU3GESHENGWUXUEYANGBENJINXINGFENXI,GENGJINGJISHIHUI;DUIYULINCHUANGYANGBENZEBUJIANYIZHEIZHONGFANGAN。

Hi-C互作测序分辨率如何理解?

Hi-CSHUJUFENBIANLVDEDAXIAO(JIbinDEDAXIAO)YOUJUTIDESHENGWUXUEWENTILAIQUEDING,YANJIUBUTONGDEWENTIYAOCAIYONGBUTONGDEFENBIANLV; JISUANFENBIANLVDEFANGFA:FENBIEANZHAOBUTONGDAXIAODEbinDUIMEIGEbinZHIJIANDEJIAOHUreadSHUMUCONGGAODAODIPAILIE,DANGPAILIEWAN80%DEbinSHI,GAIbinRENGFUGAIYOUCHAOGUO1,000TIAORead,MANZUCITIAOJIANDEZUIXIAODEbinDEDAXIAOJIWEIHi-CWENKUDEFENBIANLV。 CONGcompartmentDAOTADDAOloopSUOXUYAODEFENBIANLVDIZENG。

Hi-C互作测序是否一定要和其他组学搭配?

JIANYIHEQITAZUXUELIANHEKAIZHAN,YIBANZUISHAOHERNA-seqLIANHE,QITARUJIAJIHUACEXU、YANJIUZHUANLUYINZIJIEHEYIJIZUDANBAIXIUSHIDEchip-seq、KEYIQUESHIZENGQIANGZIQIDONGZIpeakDERANSEZHIKEJIXINGCEXUATAC-seqYIJIJIANCETUBIANDEQUANJIYINZUZHONGCEXUDENG,DOUKEYILIANHEKAIZHAN,CONGGEGECENGMIANSHUOMINGRANSEZHIGOUXIANGGAIBIANDEJIEGUOHUOYUANYIN,FENGFUCHONGSHIWENZHANGNEIRONG。DANGRAN,RUGUONINYIJINGYOUQITAZUXUESHUJU,YEKEYITIGONGJIWOMENJINXINGLIANHEFENXI。

  • 百迈客Hi-C测序的优势?

皇朝国际DA: 1)Hi-CBIAOZHUNJIANKULIUCHENGYISHENQINGGUOJIAZHUANLI,WANCHENGJIN300GEWUZHONG,JINQIANGEWENKUGOUJIAN;

皇朝国际2)BAOCHIJIN100%DEJIANKUCHENGGONGLV,WENKUHANMEIQIEWEIDIANYOUXIAOSHUJUBILIZUIGAODA93%YISHANG,PINGJUNBILIGAODA68%;

3)SHIYAN+SHENGWUXINXIFENXIZHUANLIBAOHU;

4)DUOPIANCHENGGONGANLI。

_px_2019.06.26
  • 百迈客Hi-C测序送样要求?

送样要求

样品类型 送样量 取样建议
组织 ≧1g 新鲜组织简单梯度冻存或液氮速冻,-80℃长期保存,干冰运输。
全血 ≧2mL 血液置于含 EDTA 或柠檬酸钠抗凝管中,室温平衡后立即低温送样。
细胞 ≧10^6 个 进行甲醛交联固定后,细胞或细胞核液氮速冻,-80℃长期保存,干冰运输。

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